2AG4
Crystal Structure Analysis of GM2-activator protein complexed with phosphatidylcholine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 123 |
| Detector technology | CCD |
| Collection date | 2003-08-23 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97943 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.500, 64.090, 80.990 |
| Unit cell angles | 90.00, 92.81, 90.00 |
Refinement procedure
| Resolution | 19.850 - 1.800 |
| R-factor | 0.188 |
| Rwork | 0.188 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1PU5 monomer B |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.043 | 0.315 |
| Number of reflections | 52552 | |
| <I/σ(I)> | 22 | 3.9 |
| Completeness [%] | 93.8 | 89.7 |
| Redundancy | 3.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 278 | Peg 4000, Hepes buffer, isopropanol, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
