2AF1
Structure of the DNA coiled-coil formed by d(CGATATATATAT)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM16 |
Synchrotron site | ESRF |
Beamline | BM16 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2004-11-24 |
Detector | MARRESEARCH |
Wavelength(s) | 0.972 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 26.541, 26.541, 220.527 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 23.000 - 3.100 |
R-factor | 0.33318 |
Rwork | 0.332 |
R-free | 0.34585 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gqu |
RMSD bond length | 0.008 |
RMSD bond angle | 1.980 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.000 | |
High resolution limit [Å] | 3.100 | 3.100 |
Number of reflections | 1099 | |
Completeness [%] | 99.2 | 93 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | cacodylate,MPD, sodium phosphate, potassium chloride, Trimethylamine-N-oxide, spermine , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | cacodylate | ||
10 | 1 | 2 | sodium phosphate | ||
11 | 1 | 2 | potassium chloride | ||
12 | 1 | 2 | H2O | ||
2 | 1 | 1 | MPD | ||
3 | 1 | 1 | sodium phosphate | ||
4 | 1 | 1 | potassium chloride | ||
5 | 1 | 1 | Trimethylamine-N-oxide | ||
6 | 1 | 1 | spermine | ||
7 | 1 | 1 | H2O | ||
8 | 1 | 2 | cacodylate | ||
9 | 1 | 2 | MPD |