2ADU
Human Methionine Aminopeptidase Complex with 4-Aryl-1,2,3-triazole Inhibitor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-08-01 |
Detector | MARRESEARCH |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 89.819, 99.264, 101.282 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 99.000 - 1.900 |
R-factor | 0.335 |
Rwork | 0.335 |
R-free | 0.35500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bn5 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.330 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.000 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.069 | 0.767 |
Number of reflections | 62773 | |
Completeness [%] | 90.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.4 | 274 | 100 MM CITRATE BUFFER, 30% BUTANOL, pH 5.4, VAPOR DIFFUSION, temperature 274 K, pH 5.40 |