2AD5
Mechanisms of feedback regulation and drug resistance of CTP synthetases: structure of the E. coli CTPS/CTP complex at 2.8-Angstrom resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-06-13 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.975 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 163.270, 106.380, 130.470 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| R-factor | 0.202 |
| Rwork | 0.202 |
| R-free | 0.27700 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | PDB entyry 1S1M |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.930 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | TNT (V. 5-F) |
| Refinement software | TNT (5F) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.400 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.084 | 0.310 |
| Number of reflections | 55879 | |
| <I/σ(I)> | 8.5 | 2.6 |
| Completeness [%] | 98.9 | 95.1 |
| Redundancy | 3.3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | Tris-Cl, ammonium sulfate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
