2AC5
Structure of human Mnk2 Kinase Domain mutant D228G
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-01-23 |
Detector | MARRESEARCH |
Wavelength(s) | 1.05 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 104.646, 104.646, 73.363 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 3.200 |
R-factor | 0.306 |
Rwork | 0.238 |
R-free | 0.30600 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.300 |
High resolution limit [Å] | 3.200 | 3.200 |
Number of reflections | 7776 | |
Completeness [%] | 98.2 | 99.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 290 | PEG, pH 7.5, VAPOR DIFFUSION, temperature 290K |