2ABZ

Crystal structure of C19A/C43A mutant of leech carboxypeptidase inhibitor in complex with bovine carboxypeptidase A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Collection date	2004-06-21
Spacegroup name	P 43 21 2
Unit cell lengths	124.930, 124.930, 154.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.160
R-factor	0.19
Rwork	0.189
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
Refinement software	REFMAC (5.1.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.100
Number of reflections	66322

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	1.5M Lithium Sulfate monohydrate, 100mM Tris, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K