2ABM
Crystal Structure of Aquaporin Z Tetramer Reveals both Open and Closed Water-conducting Channels
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 273 |
| Detector technology | CCD |
| Collection date | 2003-05-01 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 119.152, 119.152, 380.394 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 3.200 |
| R-factor | 0.186 |
| Rwork | 0.186 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fx8 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.260 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.012 | 0.346 |
| Number of reflections | 46497 | |
| <I/σ(I)> | 9.8 | 3.3 |
| Completeness [%] | 99.6 | 97 |
| Redundancy | 2.8 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 273 | 16% PEG3350, 0.75%beta-OG, 20% glycerol, 0.064 M Na Acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 273K |
