2A73
Human Complement Component C3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9393 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 116.980, 156.260, 271.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 3.300 |
| R-factor | 0.23302 |
| Rwork | 0.230 |
| R-free | 0.28943 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1C3D and structure of C3c |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.528 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.120 | 3.500 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.081 | 0.433 |
| Number of reflections | 37818 | |
| <I/σ(I)> | 10.7 | 2.3 |
| Completeness [%] | 99.7 | 99.2 |
| Redundancy | 4.5 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 293 | PEG 550 MME, sodium acetate, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
