2A64
Crystal Structure of Bacterial Ribonuclease P RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-07-01 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 147.150, 159.420, 410.210 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.150 - 3.300 |
| R-factor | 0.3214 |
| Rwork | 0.320 |
| R-free | 0.33990 |
| Structure solution method | MAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.800 |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 500.000 | 3.420 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.148 | 0.518 |
| Number of reflections | 35599 | |
| <I/σ(I)> | 7.5 | 3.2 |
| Completeness [%] | 99.4 | 99.9 |
| Redundancy | 5.84 | 5.49 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 298 | (NH4)2SO4, K2SO4, MgCl2, MPD, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 298 | (NH4)2SO4, K2SO4, MgCl2, MPD, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | (NH4)2SO4 | ||
| 2 | 1 | 1 | K2SO4 | ||
| 3 | 1 | 1 | MgCl2 | ||
| 4 | 1 | 1 | MPD | ||
| 5 | 1 | 2 | (NH4)2SO4 | ||
| 6 | 1 | 2 | K2SO4 | ||
| 7 | 1 | 2 | MgCl2 | ||
| 8 | 1 | 2 | MPD |
