2A5T
Crystal Structure Of The NR1/NR2A ligand-binding cores complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-01-28 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.010 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.640, 89.989, 126.116 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.950 - 2.000 |
R-factor | 0.214 |
Rwork | 0.213 |
R-free | 0.25300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 2.000 |
Number of reflections | 44066 |
Completeness [%] | 86.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 7 | 277 | PEG 8000, HEPES, Calcium acetate, pH 7., EVAPORATION, temperature 277K |