2A30
Crystal structure of human deoxycytidine kinase in complex with deoxycytidine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97626 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.337, 64.941, 108.602 |
| Unit cell angles | 90.00, 95.97, 90.00 |
Refinement procedure
| Resolution | 19.900 - 3.020 |
| R-factor | 0.24 |
| Rwork | 0.235 |
| R-free | 0.28500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p60 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.200 |
| Data reduction software | MOSFLM |
| Data scaling software | XSCALE |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.141 | 0.642 |
| Number of reflections | 20040 | 1124 |
| <I/σ(I)> | 8.77 | 2.36 |
| Completeness [%] | 83.7 | 81.2 |
| Redundancy | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | PEG 1000, MPD, Calcium Chloride, Sodium Cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
