2A2J
Crystal structure of a putative pyridoxine 5'-phosphate oxidase (Rv2607) from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 273 |
| Detector technology | CCD |
| Collection date | 2003-08-05 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.609, 91.108, 92.992 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.480 - 2.500 |
| R-factor | 0.21281 |
| Rwork | 0.209 |
| R-free | 0.26018 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g79 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.007 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.480 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 21094 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | Tris/Maleate, PEG 10000, N,N-dimethyldodecylamine N-oxide, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
