2A2C
x-ray structure of human N-acetyl galactosamine kinase complexed with Mg-ADP and N-acetyl galactosamine 1-phosphate
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-08-27 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97926, 0.97940, 0.96411 |
Spacegroup name | P 65 |
Unit cell lengths | 123.800, 123.800, 60.100 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 1.650 |
R-factor | 0.168 |
Rwork | 0.167 |
R-free | 0.20100 |
Structure solution method | MAD |
RMSD bond length | 0.013 |
RMSD bond angle | 2.370 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | TNT |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.710 |
High resolution limit [Å] | 1.650 | 1.650 |
Number of reflections | 59826 | |
<I/σ(I)> | 46.3 | 3.5 |
Completeness [%] | 94.3 | 72.7 |
Redundancy | 8.6 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | PEG-3400, MES, NaCl, Mg-ATP, N-acetyl galactosamine, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |