2A27
Human DRP-1 kinase, W305S S308A D40 mutant, crystal form with 8 monomers in the asymmetric unit
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-09-11 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8416 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 159.120, 113.350, 247.310 |
| Unit cell angles | 90.00, 95.09, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.000 |
| R-factor | 0.21852 |
| Rwork | 0.217 |
| R-free | 0.23844 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.142 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 3.200 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 87689 | |
| <I/σ(I)> | 11.4 | 2.8 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 295 | ammonium sulfate, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
