2A0U
Crystal structure of the eukaryotic initiation factor 2B from Leishmania major at 2.1 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.979359 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 103.733, 164.569, 172.129 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.300 - 2.100 |
| R-factor | 0.21631 |
| Rwork | 0.214 |
| R-free | 0.26378 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.989 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.300 | 2.213 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 34856 | |
| <I/σ(I)> | 8.7 | 1.6 |
| Completeness [%] | 85.4 | 63.13 |
| Redundancy | 2.9 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 2.1 M Sodium thiosulfate pentahydrate,0.1 M MOPS 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
