2A07
Crystal Structure of Foxp2 bound Specifically to DNA.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2004-06-04 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.07810 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.542, 124.210, 67.666 |
| Unit cell angles | 90.00, 110.81, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.900 |
| R-factor | 0.2224 |
| Rwork | 0.222 |
| R-free | 0.24360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.067 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.055 | |
| Number of reflections | 79203 | |
| <I/σ(I)> | 28.46 | |
| Completeness [%] | 96.4 | 8.4 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.68 | 291 | BTP, Sodium Chloride, PEG 3k, Magnesium Chloride, Sodium Azide, pH 6.68, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | BTP | ||
| 10 | 1 | 2 | H2O | ||
| 2 | 1 | 1 | Sodium Chloride | ||
| 3 | 1 | 1 | PEG 3k | ||
| 4 | 1 | 1 | Magnesium Chloride | ||
| 5 | 1 | 1 | Sodium Azide | ||
| 6 | 1 | 1 | H2O | ||
| 7 | 1 | 2 | Sodium Chloride | ||
| 8 | 1 | 2 | PEG 3k | ||
| 9 | 1 | 2 | Magnesium Chloride |
