28YJ
Molecular basis of ZPD homopolymerization: cryo-EM structure of a native vertebrate egg coat filament
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | TFS Selectris |
| Detector | FEI FALCON IV (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.6 |
| Number of Particle | 1165059 |
| Software | cryoSPARC,EMReady,REFMAC,Servalcat,PHENIX,EPU,UCSF Chimera,ISOLDE,Coot |
| Symmetry | C1 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
