28MS
X-ray structure of the adduct between human serum transferrin with Fe3+ bound at the C-lobe and dirhodium tetraacetate
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-04-10 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.98011 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 137.776, 157.085, 107.559 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 103.580 - 2.370 |
| Rwork | 0.183 |
| R-free | 0.24430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.781 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 103.580 | 2.604 |
| High resolution limit [Å] | 2.368 | 2.368 |
| Rmerge | 0.205 | 2.088 |
| Number of reflections | 33787 | 1689 |
| <I/σ(I)> | 10.3 | 1.5 |
| Completeness [%] | 92.8 | 58.1 |
| Redundancy | 13.4 | 11.4 |
| CC(1/2) | 0.996 | 0.581 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 15% (w/v) PEG 3350 16% (v/v) glycerol 8 mM disodium malonate 150 mM Na-PIPES pH 6.5 |
