24BQ

Crystal structure of nuclease MYG1(H50A) bound to Mn2+ and dAC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL18U
Synchrotron site	NFPSS
Beamline	BL18U
Temperature [K]	193
Detector technology	PIXEL
Collection date	2025-03-22
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9786
Spacegroup name	C 2 2 21
Unit cell lengths	70.346, 97.719, 117.066
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.560 - 2.300
R-factor	0.1926
Rwork	0.187
R-free	0.23950
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.811
Data reduction software	HKL-2000 (1.20.1_4487)
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.560	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	18081	1705
<I/σ(I)>	3.5
Completeness [%]	99.1
Redundancy	3.3
CC(1/2)	0.910	0.750

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.2 M lithium sulfate, 0.1 M Tris, pH 5.5, 25% w/v PEG 3350