22CR
C5a-desArg bound C5aR2 in complex with beta-arrestin1
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | FEI FALCON IV (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.9 |
| Software | RELION,cryoSPARC |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
