21NX
Cryo-EM structure of mouse IgG2b-Fc in complex with FcgammaRIV
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | FEI POLARA 300 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | FEI FALCON IV (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.99 |
| Software | cryoSPARC,PHENIX |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
