21BQ
Cryo-EM structure of ex vivo Sup35 prion fibrils from yeast carrying "strong" [PSI+] variant
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 0.01 |
| Energy Filter | GIF Bioquantum |
| Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 2.7 |
| Number of Particle | 1259000 |
| Software | crYOLO,cryoSPARC,PHENIX,SerialEM,CTFFIND |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
