1ZVR
Crystal Structure of MTMR2 in complex with phosphatidylinositol 3,5-bisphosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-04-27 |
Detector | MARRESEARCH |
Wavelength(s) | 1.009 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 66.180, 66.180, 262.370 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.980 |
Rwork | 0.228 |
R-free | 0.25200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ID 1LW3 |
RMSD bond length | 0.032 |
RMSD bond angle | 2.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.100 |
High resolution limit [Å] | 1.980 | 1.980 |
Number of reflections | 40385 | |
<I/σ(I)> | 32.3 | 2.1 |
Completeness [%] | 96.7 | 63.4 |
Redundancy | 12 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | PEG 10,000, Tris, TCEP, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |