1ZVF

The crystal structure of 3-hydroxyanthranilate 3,4-dioxygenase from Saccharomyces cerevisiae

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Wavelength(s)	0.979, 0.9798, 0.9802, 0.9500
Spacegroup name	P 21 21 21
Unit cell lengths	41.104, 66.195, 130.188
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.410
R-factor	0.2203
Rwork	0.217
R-free	0.26750
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.310
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.440
High resolution limit [Å]	2.400	6.490	2.400
Rmerge	0.082	0.065	0.244
Number of reflections	13576
Completeness [%]	93.2
Redundancy	12.3	11.6	9.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	hanging drop	8.5	293	30% PEG4000, 0.2M NaAc, 0.1M Tris_HCl, pH 8.5, hanging drop, temperature 293K
1	hanging drop	8.5	293	30% PEG4000, 0.2M NaAc, 0.1M Tris_HCl, pH 8.5, hanging drop, temperature 293K
1	hanging drop	8.5	293	30% PEG4000, 0.2M NaAc, 0.1M Tris_HCl, pH 8.5, hanging drop, temperature 293K