1ZVD
Regulation of Smurf2 Ubiquitin Ligase Activity by Anchoring the E2 to the HECT domain
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-D |
Synchrotron site | APS |
Beamline | 14-BM-D |
Temperature [K] | 200 |
Collection date | 2002-04-12 |
Wavelength(s) | 0.9799, 0.9997, 0.9568 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.359, 70.509, 152.351 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.570 - 2.100 |
Rwork | 0.202 |
R-free | 0.24792 |
Structure solution method | MAD |
RMSD bond length | 0.023 |
RMSD bond angle | 1.883 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 24.600 |
High resolution limit [Å] | 2.100 |
Number of reflections | 32394 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 298 | Sodium Phosphate, Potassium Phosphate, and Sodium Acetate, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |