1ZV8
A structure-based mechanism of SARS virus membrane fusion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-09-03 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9788 |
Spacegroup name | P 1 |
Unit cell lengths | 31.054, 55.565, 71.678 |
Unit cell angles | 81.26, 87.08, 84.10 |
Refinement procedure
Resolution | 70.710 - 1.940 |
R-factor | 0.20699 |
Rwork | 0.203 |
R-free | 0.27427 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.033 |
RMSD bond angle | 2.264 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.700 | 2.020 |
High resolution limit [Å] | 1.940 | 1.950 |
Rmerge | 0.055 | 0.387 |
Number of reflections | 32684 | |
<I/σ(I)> | 11.1 | 3.1 |
Completeness [%] | 94.5 | 94.8 |
Redundancy | 2.5 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 298 | PEG 8000, zinc acetate, sodium cacodylate, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |