1ZT4
The crystal structure of human CD1d with and without alpha-Galactosylceramide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-10-11 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9340 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.470, 94.300, 176.070 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 3.000 |
R-factor | 0.234 |
Rwork | 0.234 |
R-free | 0.32600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1cd1 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.650 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.190 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.184 | 0.940 |
Number of reflections | 17523 | |
<I/σ(I)> | 15.2 | 3.2 |
Completeness [%] | 99.8 | 100 |
Redundancy | 11.2 | 11.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | PEG3350, potassium fluoride, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K |