1ZS8

Crystal Structure of the Murine MHC Class Ib Molecule M10.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 12.3.1
Synchrotron site	ALS
Beamline	12.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-04-03
Detector	ADSC QUANTUM 315
Wavelength(s)	1.11587
Spacegroup name	P 21 21 21
Unit cell lengths	124.110, 134.710, 149.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.360 - 3.000
R-factor	0.307
Rwork	0.307
R-free	0.30800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1K8D (confirmed with 3FRU)
RMSD bond length	0.009
RMSD bond angle	1.600
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000	3.110
High resolution limit [Å]	3.000	3.000
Rmerge	0.156	0.535
Number of reflections	46977
<I/σ(I)>	9	2.7
Completeness [%]	91.2	64.8
Redundancy	5.7	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	0.1 M imidazole, 20% PEG 1000, 0.2 M calcium acetate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	8	298	0.1 M imidazole, 20% PEG 1000, 0.2 M calcium acetate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K