1ZR3

Crystal structure of the macro-domain of human core histone variant macroH2A1.1 (form B)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2005-02-05
Detector	ADSC QUANTUM 4
Wavelength(s)	0.953720
Spacegroup name	P 21 21 21
Unit cell lengths	80.900, 89.200, 92.730
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	64.280 - 1.660
R-factor	0.19034
Rwork	0.188
R-free	0.23166
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDP ID 1ZQ0
RMSD bond length	0.021
RMSD bond angle	1.792
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.280	1.760
High resolution limit [Å]	1.660	1.660
Number of reflections	78100
<I/σ(I)>	21.06	2.41
Completeness [%]		86.4
Redundancy	7.2	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microbridge	6.5	298	22% PEG 1000 0.1 M Mes pH6.5, Microbridge, temperature 298K