1ZO9
Crystal Structure Of The Wild Type Heme Domain Of P450BM-3 with N-palmitoylmethionine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-10-28 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.039, 147.807, 63.604 |
| Unit cell angles | 90.00, 98.60, 90.00 |
Refinement procedure
| Resolution | 29.600 - 1.700 |
| R-factor | 0.199 |
| Rwork | 0.164 |
| R-free | 0.19900 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1bu7 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.570 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.600 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.029 | 0.368 |
| Number of reflections | 112549 | |
| <I/σ(I)> | 37.4 | 1.4 |
| Completeness [%] | 79.2 | 13.1 |
| Redundancy | 2.7 | 1.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | PEG 3350, MES, Magnesium Chloride, Glycerol, pH 6.0, temperature 277K, VAPOR DIFFUSION, HANGING DROP |
