1ZNB
METALLO-BETA-LACTAMASE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1995-05-02 |
| Detector | SIEMENS |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.298, 78.298, 140.916 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.850 |
| R-factor | 0.167 |
| Rwork | 0.167 |
| R-free | 0.24100 |
| Structure solution method | MULTIWAVELENGTH ANOMALOUS DIFFRACTION (MAD): |
| RMSD bond length | 0.020 |
| RMSD bond angle | 24.400 * |
| Data reduction software | XENGEN |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 1.970 |
| High resolution limit [Å] | 1.815 | 1.850 |
| Rmerge | 0.077 * | 0.398 * |
| Total number of observations | 292398 * | |
| Number of reflections | 37905 | 5995 * |
| <I/σ(I)> | 6.7 | 1.7 |
| Completeness [%] | 99.2 | 95.8 |
| Redundancy | 7.7 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | pH 7.0 | |
| 1 | Vapor diffusion, hanging drop * | 7 | pH 7.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5-8 (mg/ml) | |
| 2 | 1 | drop | HEPES | 55 (mM) | |
| 3 | 1 | drop | 0.01 (mM) | ||
| 4 | 1 | drop | PEG2000 | 26-28 (%) | |
| 5 | 1 | reservoir | PEG2000 | 26-28 (%) | |
| 6 | 1 | reservoir | HEPES | 0.1 (M) |
