1ZMD
Crystal Structure of Human dihydrolipoamide dehydrogenase complexed to NADH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-07-10 |
Detector | APS |
Wavelength(s) | 0.97943 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 175.871, 210.914, 127.096 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.060 - 2.080 |
R-factor | 0.221 |
Rwork | 0.221 |
R-free | 0.24900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jeh |
RMSD bond length | 0.013 |
RMSD bond angle | 1.600 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.100 | 2.150 |
High resolution limit [Å] | 2.080 | 2.080 |
Number of reflections | 281013 | |
<I/σ(I)> | 20.1 | 3.3 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 8.3 | 7.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | HEPES, PEG 4000, ammonium sulfate, pH 7.0, temperature 293K, VAPOR DIFFUSION, HANGING DROP |