1ZMD
Crystal Structure of Human dihydrolipoamide dehydrogenase complexed to NADH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-10 |
| Detector | APS |
| Wavelength(s) | 0.97943 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 175.871, 210.914, 127.096 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.060 - 2.080 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jeh |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.100 | 2.150 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Number of reflections | 281013 | |
| <I/σ(I)> | 20.1 | 3.3 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 8.3 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | HEPES, PEG 4000, ammonium sulfate, pH 7.0, temperature 293K, VAPOR DIFFUSION, HANGING DROP |
