1ZMC

Crystal Structure of Human dihydrolipoamide dehydrogenase complexed to NAD+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2004-08-15
Detector	APS
Wavelength(s)	0.979351
Spacegroup name	P 21 21 2
Unit cell lengths	176.124, 210.763, 126.903
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.090 - 2.530
R-factor	0.211
Rwork	0.211
R-free	0.26700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jeh
RMSD bond length	0.011
RMSD bond angle	1.600
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.100	2.620
High resolution limit [Å]	2.530	2.530
Number of reflections	156541
<I/σ(I)>	16.5	2.6
Completeness [%]	99.3	96.5
Redundancy	4	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	HEPES, PEG 4000, ammonium sulfate, pH 7.0, temperature 293K, VAPOR DIFFUSION, HANGING DROP