1ZM9
Structure of eEF2-ETA in complex with PJ34
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Wavelength(s) | 0.976 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 330.840, 68.740, 191.460 |
Unit cell angles | 90.00, 103.50, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.800 |
R-factor | 0.255 |
Rwork | 0.254 |
R-free | 0.27400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | pdb entries 1n0u and 1aer |
RMSD bond length | 0.010 |
RMSD bond angle | 1.430 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.150 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 103348 | |
<I/σ(I)> | 9.7 | |
Completeness [%] | 99.2 | 98.8 |
Redundancy | 3.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 293 | PEG 6000, MPD, HEPES, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |