1ZL3
Coupling of active site motions and RNA binding
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-03-07 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 145.433, 40.753, 78.803 |
Unit cell angles | 90.00, 110.61, 90.00 |
Refinement procedure
Resolution | 19.920 - 2.800 |
R-factor | 0.202 |
Rwork | 0.202 |
R-free | 0.26400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1k8w |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.091 | 0.341 |
Number of reflections | 10935 | |
<I/σ(I)> | 23.6 | 3.6 |
Completeness [%] | 99.9 | 99.4 |
Redundancy | 6.34 | 4.71 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | ammonium sulfate [1.7M], PEG400 [2%], Hepes-KOH pH 7.5 [0.1M] and TCEP [1 mM], pH 7.50, VAPOR DIFFUSION, SITTING DROP, temperature 298K |