1ZL2
Crystal structure of the uridine phosphorylase from Salmonella typhimurium in complex with 2,2'-anhydrouridine and phosphate ion at 1.85A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.803 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 88.790, 124.070, 134.110 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.090 - 1.850 |
| R-factor | 0.178 |
| Rwork | 0.178 |
| R-free | 0.19800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sj9 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.400 |
| Data scaling software | XDS |
| Phasing software | MOLREP (V. 7.5.01) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.090 | 1.970 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.123 | 0.399 |
| Number of reflections | 120586 | |
| <I/σ(I)> | 11.24 | 3.55 |
| Completeness [%] | 95.1 | 96.2 |
| Redundancy | 3.25 | 3.25 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 293 | PEG 4000, SODIUM-ACETATE TRIHYDRATE, 2,2'-ANHYDROURIDINE, SODIUM PHOSPHATE, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
