1ZKK
Crystal structure of hSET8 in ternary complex with H4 peptide (16-24) and AdoHcy
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 32-ID |
Synchrotron site | APS |
Beamline | 32-ID |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2004-10-31 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9686, 0.9792, 0.9791 |
Spacegroup name | P 1 |
Unit cell lengths | 43.960, 45.775, 94.435 |
Unit cell angles | 89.22, 87.07, 90.72 |
Refinement procedure
Resolution | 19.630 - 1.450 |
R-factor | 0.17073 |
Rwork | 0.169 |
R-free | 0.19902 |
Structure solution method | MAD, MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.278 |
Data scaling software | XDS |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 22.000 | 1.500 |
High resolution limit [Å] | 1.450 | 1.450 |
Number of reflections | 122425 | |
Completeness [%] | 32.0 | 34 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | pentaerythritol ethoxylate, ammonium sulfate, Bis-Tris, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |