1ZJM
Human DNA Polymerase beta complexed with DNA containing an A-A mismatched primer terminus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-11-12 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.100, 79.100, 54.900 |
Unit cell angles | 90.00, 105.90, 90.00 |
Refinement procedure
Resolution | 30.330 - 2.100 |
R-factor | 0.228 |
Rwork | 0.228 |
R-free | 0.23400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bpx |
RMSD bond length | 0.005 |
RMSD bond angle | 1.000 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.081 | 0.436 |
Number of reflections | 24584 | |
Completeness [%] | 99.2 | 92.3 |
Redundancy | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | PEG 3350, Sodium Acetate, Imidazole, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Sodium Acetate | ||
2 | 1 | 1 | Imidazole | ||
3 | 1 | 1 | PEG 3350 | ||
4 | 1 | 2 | Sodium Acetate | ||
5 | 1 | 2 | PEG 3350 |