1ZIW

Human Toll-like Receptor 3 extracellular domain structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-03-15
Detector	ADSC QUANTUM 4
Wavelength(s)	1.07812
Spacegroup name	C 2 2 21
Unit cell lengths	138.497, 155.121, 119.669
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.100
R-factor	0.2103
Rwork	0.208
R-free	0.24790
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.641
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0013)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.170
High resolution limit [Å]	2.100	2.100
Rmerge	0.062	0.565
Number of reflections	76420
<I/σ(I)>	20	2.2
Completeness [%]	99.7	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	295	PEG2000, Ammonium Sulfate, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
1	VAPOR DIFFUSION, SITTING DROP	7	295	PEG2000, Ammonium Sulfate, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
1	VAPOR DIFFUSION, SITTING DROP	7	295	PEG2000, Ammonium Sulfate, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K