1ZIS
Recombinant Lumazine synthase (hexagonal form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM16 |
| Synchrotron site | ESRF |
| Beamline | BM16 |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 2004-06-22 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 153.690, 153.690, 296.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.900 |
| Rwork | 0.206 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pentamer from 1RVV |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.600 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.085 | 0.190 |
| Number of reflections | 40931 | |
| <I/σ(I)> | 17.59 | 8.38 |
| Completeness [%] | 87.5 | 77.1 |
| Redundancy | 7.3 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.7 | 293 | Phosphate buffer, pH 8.7, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
