1ZHX
Structure of yeast oxysterol binding protein Osh4 in complex with 25-hydroxycholesterol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2004-08-26 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 82.600, 95.170, 65.570 |
Unit cell angles | 90.00, 96.81, 90.00 |
Refinement procedure
Resolution | 47.110 - 1.500 |
R-factor | 0.22 |
Rwork | 0.220 |
R-free | 0.23300 |
Structure solution method | MIR |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.047 | 0.200 |
Number of reflections | 78130 | |
<I/σ(I)> | 54.7 | 7.9 |
Completeness [%] | 97.6 | 92.5 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | PEG 20000, MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |