1ZHX
Structure of yeast oxysterol binding protein Osh4 in complex with 25-hydroxycholesterol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2004-08-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 82.600, 95.170, 65.570 |
| Unit cell angles | 90.00, 96.81, 90.00 |
Refinement procedure
| Resolution | 47.110 - 1.500 |
| R-factor | 0.22 |
| Rwork | 0.220 |
| R-free | 0.23300 |
| Structure solution method | MIR |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.047 | 0.200 |
| Number of reflections | 78130 | |
| <I/σ(I)> | 54.7 | 7.9 |
| Completeness [%] | 97.6 | 92.5 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | PEG 20000, MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
