1ZH6
Crystal Structure of p-acetylphenylalanine-tRNA synthetase in complex with p-acetylphenylalanine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-07-31 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 103.362, 103.362, 70.837 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 73.130 - 2.500 |
R-factor | 0.213 |
Rwork | 0.209 |
R-free | 0.27773 |
Structure solution method | isomorphous with 1ZH0 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.373 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CCP4 |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.096 | 0.557 |
Number of reflections | 13751 | |
<I/σ(I)> | 17.9 | 2.2 |
Completeness [%] | 99.1 | 97.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 20% PEG-300, 5% PEG-8000,10% glycerol 0.1M Tris, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |