1ZEH

STRUCTURE OF INSULIN

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	1997-01-27
Detector	MARRESEARCH
Spacegroup name	H 3
Unit cell lengths	77.490, 77.490, 38.910
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.390 * - 1.510 *
R-factor	0.159 *
Rwork	0.159
R-free	0.19300
Starting model (for MR)	4ZN INSULIN DIMER
RMSD bond length	0.014
RMSD bond angle	0.036
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.390 *	1.530
High resolution limit [Å]	1.510 *	1.510 *
Rmerge	0.070	0.169
Total number of observations	102174 *
Number of reflections	13601
Completeness [%]	97.5	54.6
Redundancy	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	batch method	6.8	50 *	BATCH METHOD, COMPOSITION OF CRYSTALLISATION SOLUTION 3.5 MG INSULIN + 0.5 ML 0.02M HCL + 0.05 ML 0.12M ZINC ACETATE + 0.25 ML 0.2M TRI-SODIUM CITRATE + 0.2 ML 2.5% (W/V) M-CRESOL IN ETHANOL + 60 MG NACL, pH 6.8, batch method

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	insulin	3.5 (mg/ml)
2	1	1		10 (mM)
3	1	1	zinc acetate	6 (mM)
4	1	1	trisodium citrate	5 (mM)
5	1	1	m-cresol in ethanol	0.5 (%)
6	1	1		60 (mg/ml)