1ZEH
STRUCTURE OF INSULIN
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-01-27 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 |
| Unit cell lengths | 77.490, 77.490, 38.910 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.390 * - 1.510* |
| R-factor | 0.159 * |
| Rwork | 0.159 |
| R-free | 0.19300 |
| Starting model (for MR) | 4ZN INSULIN DIMER |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.036 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.390 * | 1.530 |
| High resolution limit [Å] | 1.510 * | 1.510 * |
| Rmerge | 0.070 | 0.169 |
| Total number of observations | 102174 * | |
| Number of reflections | 13601 | |
| Completeness [%] | 97.5 | 54.6 |
| Redundancy | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | batch method | 6.8 | 50 * | BATCH METHOD, COMPOSITION OF CRYSTALLISATION SOLUTION 3.5 MG INSULIN + 0.5 ML 0.02M HCL + 0.05 ML 0.12M ZINC ACETATE + 0.25 ML 0.2M TRI-SODIUM CITRATE + 0.2 ML 2.5% (W/V) M-CRESOL IN ETHANOL + 60 MG NACL, pH 6.8, batch method |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | insulin | 3.5 (mg/ml) | |
| 2 | 1 | 1 | 10 (mM) | ||
| 3 | 1 | 1 | zinc acetate | 6 (mM) | |
| 4 | 1 | 1 | trisodium citrate | 5 (mM) | |
| 5 | 1 | 1 | m-cresol in ethanol | 0.5 (%) | |
| 6 | 1 | 1 | 60 (mg/ml) |
