1ZE1
Conformational Change of Pseudouridine 55 Synthase upon Its Association with RNA Substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.650, 64.170, 176.070 |
| Unit cell angles | 87.48, 86.44, 79.35 |
Refinement procedure
| Resolution | 50.000 - 2.900 |
| Rwork | 0.235 |
| R-free | 0.28100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ze2 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.270 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.900 |
| Number of reflections | 40146 |
| <I/σ(I)> | 14.2 |
| Completeness [%] | 94.5 |
| Redundancy | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | ||||
| 1 |
