1ZDK
STRUCTURE OF BACTERIOPHAGE COAT PROTEIN-LOOP RNA COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID2 |
| Synchrotron site | ESRF |
| Beamline | ID2 |
| Temperature [K] | 280 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-12-12 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 288.000, 288.000, 653.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.860 |
| R-factor | 0.207 * |
| Rwork | 0.207 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.130 | 0.247 |
| Number of reflections | 149789 | |
| Completeness [%] | 47.4 | 6.5 |
| Redundancy | 1.7 | 1.07 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.4 | 37 * | Valegard, K., (1986) J. Mol. Biol., 190, 587. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | MS2 | 1.0 (%(w/v)) | |
| 2 | 1 | drop | sodium phosphate | 0.2 (M) | |
| 3 | 1 | drop | PEG6000 | 1.5 (%(w/v)) | |
| 4 | 1 | drop | 0.02 (%(w/v)) | ||
| 5 | 1 | reservoir | sodium phospahte | 0.4 (M) |
