1ZDK

STRUCTURE OF BACTERIOPHAGE COAT PROTEIN-LOOP RNA COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID2
Synchrotron site	ESRF
Beamline	ID2
Temperature [K]	280
Detector technology	IMAGE PLATE
Collection date	1994-12-12
Detector	MARRESEARCH
Spacegroup name	H 3 2
Unit cell lengths	288.000, 288.000, 653.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.860
R-factor	0.207 *
Rwork	0.207
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.018
RMSD bond angle	1.800
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.130	0.247
Number of reflections	149789
Completeness [%]	47.4	6.5
Redundancy	1.7	1.07

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.4	37 *	Valegard, K., (1986) J. Mol. Biol., 190, 587. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	MS2	1.0 (%(w/v))
2	1	drop	sodium phosphate	0.2 (M)
3	1	drop	PEG6000	1.5 (%(w/v))
4	1	drop		0.02 (%(w/v))
5	1	reservoir	sodium phospahte	0.4 (M)