1ZCL
prl-1 c104s mutant in complex with sulfate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F2 |
| Synchrotron site | CHESS |
| Beamline | F2 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2004-11-20 |
| Detector | RIGAKU AFC-5 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 146.681, 146.681, 146.681 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.900 |
| Rwork | 0.261 |
| R-free | 0.29600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rxd |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 10372 | |
| Completeness [%] | 88.1 | 99.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 291 | Ammonium Sulfate, Sodium Acetate, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
