1ZCD
Crystal structure of the Na+/H+ antiporter NhaA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-02-09 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9717 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 108.868, 121.718, 123.578 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 3.450 |
R-factor | 0.3016 |
Rwork | 0.316 |
R-free | 0.31600 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.573 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.570 |
High resolution limit [Å] | 3.450 | 3.450 |
Number of reflections | 19993 | |
<I/σ(I)> | 30.5 | |
Completeness [%] | 91.2 | 64.1 |
Redundancy | 18.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 279 | PEG 400, MgCl2, KCl, citrate, VAPOR DIFFUSION, HANGING DROP, temperature 279K |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 279 | PEG 400, MgCl2, KCl, citrate, VAPOR DIFFUSION, HANGING DROP, temperature 279K |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 279 | PEG 400, MgCl2, KCl, citrate, VAPOR DIFFUSION, HANGING DROP, temperature 279K |