1ZCA
Crystal structure of G alpha 12 in complex with GDP, Mg2+ and AlF4-
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-12-14 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.501, 85.230, 82.885 |
Unit cell angles | 90.00, 106.09, 90.00 |
Refinement procedure
Resolution | 29.100 - 2.900 |
R-factor | 0.23895 |
Rwork | 0.236 |
R-free | 0.29608 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zcb |
RMSD bond length | 0.007 |
RMSD bond angle | 1.019 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.100 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 15297 | |
<I/σ(I)> | 6.9 | 1.67 |
Completeness [%] | 88.7 | |
Redundancy | 2.2 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 297 | PEG 8000 (14%), Sodium Citrate (100 mM), NaCl (50 mM), pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K |