1ZBQ
Crystal Structure Of Human 17-Beta-Hydroxysteroid Dehydrogenase Type 4 In Complex With NAD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-13 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.91840 |
| Spacegroup name | P 1 |
| Unit cell lengths | 50.826, 50.771, 183.775 |
| Unit cell angles | 87.52, 87.07, 70.75 |
Refinement procedure
| Resolution | 50.570 - 2.190 |
| R-factor | 0.1905 |
| Rwork | 0.188 |
| R-free | 0.23568 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gz6 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.347 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.574 | 2.247 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Number of reflections | 64535 | |
| Completeness [%] | 76.2 | 29.71 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.2M Lithium Sulphate monohydrate, 0.1M TRIS hydrochloride pH8.5, 30% w/v polyethylene glycol 4000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
