1ZB7
Crystal Structure of Botulinum Neurotoxin Type G Light Chain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-12-10 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.00863 |
Spacegroup name | P 62 2 2 |
Unit cell lengths | 178.866, 178.866, 80.864 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.810 - 2.350 |
R-factor | 0.17364 |
Rwork | 0.174 |
R-free | 0.22216 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1f82 |
RMSD bond length | 0.027 |
RMSD bond angle | 2.031 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0011) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.411 |
High resolution limit [Å] | 2.350 | 2.350 |
Number of reflections | 30233 | |
<I/σ(I)> | 24.4 | 4.8 |
Completeness [%] | 93.9 | 95.3 |
Redundancy | 7.5 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 6.00% PEG 6000, 0.65M Lithium Chloride, 0.10M Sodium Citrate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |